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object --+
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type --+
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RestrictionType --+
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AbstractCut --+
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Ov3
Implement the methods specific to the enzymes for which the overhang is recessed in 5'.
The enzyme cuts the - strand after the + strand of the DNA.
Internal use only. Not meant to be instantiated.
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Inherited from Inherited from Inherited from |
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| tuple of DNA |
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| tuple of DNA |
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| bool |
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| bool |
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| bool |
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| str |
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| list |
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| bool |
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Inherited from |
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Inherited from Inherited from |
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RE.catalyze(dna, linear=True) -> tuple of DNA. return a tuple of dna as will be produced by using RE to restrict the dna. dna must be a Bio.Seq.Seq instance or a Bio.Seq.MutableSeq instance. if linear is False, the sequence is considered to be circular and the output will be modified accordingly.
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RE.catalyze(dna, linear=True) -> tuple of DNA. return a tuple of dna as will be produced by using RE to restrict the dna. dna must be a Bio.Seq.Seq instance or a Bio.Seq.MutableSeq instance. if linear is False, the sequence is considered to be circular and the output will be modified accordingly.
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True if the enzyme produces blunt end.
see also :
RE.is_3overhang()
RE.is_5overhang()
RE.is_unknown()
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True if the enzyme produces 5' overhang sticky end.
see also :
RE.is_3overhang()
RE.is_blunt()
RE.is_unknown()
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True if the enzyme produces 3' overhang sticky end.
see also :
RE.is_5overhang()
RE.is_blunt()
RE.is_unknown()
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type of overhang of the enzyme., can be "3' overhang", "5' overhang", "blunt", "unknown"
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for internal use only test for the compatibility of restriction ending of RE and other.
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